Applications of nuclear reprogramming and directed differentiation in vascular regenerative medicine

N Biotechnol. 2015 Jan 25;32(1):191-8. doi: 10.1016/j.nbt.2014.07.005. Epub 2014 Jul 23.

Abstract

As vertebrates proceed through embryonic development the growing organism cannot survive on diffusion of oxygen and nutrients alone and establishment of vascular system is fundamental for embryonic development to proceed. Dysfunction of the vascular system in adults is at the heart of many disease states such as hypertension and atherosclerosis. In this review we will focus on attempts to generate the key cells of the vascular system, the endothelial and smooth muscle cells, using human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). Regardless of their origin, be it embryonic or via somatic cell reprogramming, pluripotent stem cells provide limitlessly self-renewing populations of material suitable for the generation of multi-lineage isogenic vascular cells-types that can be used as tools to study normal cell and tissue biology, model disease states and also as tools for drug screening and future cell therapies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Blood Vessels / physiology*
  • Cell Differentiation*
  • Cellular Reprogramming*
  • Endothelial Cells / cytology
  • Humans
  • Myocytes, Smooth Muscle / cytology
  • Regenerative Medicine / methods*