Reversible ADP-ribosylation of dinitrogenase reductase in a nifD- mutant of Rhodospirillum rubrum

J Bacteriol. 1989 Sep;171(9):5210-1. doi: 10.1128/jb.171.9.5210-5211.1989.

Abstract

Dinitrogenase reductase from a Rhodospirillum rubrum strain lacking dinitrogenase was reversibly ADP-ribosylated in vivo in response to dark-light transitions. Addition of ammonia also led to ADP-ribosylation in this strain. These results demonstrate that reduced dinitrogenase is a satisfactory substrate for the reversible ADP-ribosylation system of R. rubrum in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenosine Diphosphate Ribose / metabolism
  • Darkness
  • Dinitrogenase Reductase
  • Electrophoresis, Gel, Two-Dimensional
  • Enzyme Repression
  • Ferredoxins / isolation & purification
  • Ferredoxins / metabolism*
  • Genes, Bacterial*
  • Light
  • Mutation*
  • Nitrogen Fixation / genetics*
  • Nitrogenase / biosynthesis
  • Rhodospirillum rubrum / genetics
  • Rhodospirillum rubrum / metabolism*

Substances

  • Dinitrogenase Reductase
  • Ferredoxins
  • Adenosine Diphosphate Ribose
  • Nitrogenase