Engineering enzyme-cleavable hybrid click capsules with a pH-sheddable coating for intracellular degradation

Sylvia T Gunawan; Kang Liang; Georgina K Such; Angus P R Johnston; Melissa K M Leung; Jiwei Cui; Frank Caruso

doi:10.1002/smll.201400450

Engineering enzyme-cleavable hybrid click capsules with a pH-sheddable coating for intracellular degradation

Small. 2014 Oct 29;10(20):4080-6. doi: 10.1002/smll.201400450. Epub 2014 Jul 10.

Authors

Sylvia T Gunawan¹, Kang Liang, Georgina K Such, Angus P R Johnston, Melissa K M Leung, Jiwei Cui, Frank Caruso

Affiliation

¹ Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria, 3010, Australia.

PMID: 25044500
DOI: 10.1002/smll.201400450

Abstract

The engineering of layer-by-layer (LbL) hybrid click capsules that are responsive to biological stimuli is reported. The capsules comprise a pH-sheddable, non cross-linked outer coating that protects enzyme-cleavable inner layers. Upon cellular uptake, the outer coating is released and the capsules are enzymatically degraded. In vitro cell degradation results in rapid capsule degradation (10 min) upon cellular internalization.

Keywords: click chemistry; enzyme-responsive; layer-by-layer (LbL); nanotechnology; polymer capsules.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Capsules*
Click Chemistry
Enzymes / metabolism*
Hydrogen-Ion Concentration*
Microscopy, Electron, Transmission
Polymers / chemistry

Substances

Capsules
Enzymes
Polymers