Abstract
Objective:
To detect the expressions of Toll-like receptor 2 (TLR2), TLR7, TLR9 and dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) mRNA and protein in peripheral blood mononuclear cells (PBMCs) from patients with chronic urticaria (CU).
Methods:
PBMCs were isolated from 20 patients with CU and 20 normal controls. The expressions of TLR2, TLR7, TLR9 and DC-SIGN mRNA were detected by real-time fluorescent quantitative PCR and the expressions of their proteins were detected by flow cytometry.
Results:
The expression of DC-SIGN mRNA in PBMCs from patients with CU was significantly lower than that from normal controls (P<0.01). The expression of TLR2 protein was significantly higher (P<0.05), but the expression of DC-SIGN protein was significantly lower (P<0.05) in CU patients than those in normal controls.
Conclusion:
The expression of DC-SIGN decreases and that of TLR2 protein increases in patients with CU.
MeSH terms
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Adult
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Cell Adhesion Molecules / genetics*
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Cell Adhesion Molecules / metabolism
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Cells, Cultured
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Chronic Disease
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Female
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Flow Cytometry
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Gene Expression*
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Humans
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Lectins, C-Type / genetics*
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Lectins, C-Type / metabolism
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Leukocytes, Mononuclear / metabolism*
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Male
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Middle Aged
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Receptors, Cell Surface / genetics*
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Receptors, Cell Surface / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Toll-Like Receptor 2 / genetics
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Toll-Like Receptor 2 / metabolism
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Toll-Like Receptor 7 / genetics
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Toll-Like Receptor 7 / metabolism
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Toll-Like Receptor 9 / genetics
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Toll-Like Receptor 9 / metabolism
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Toll-Like Receptors / genetics*
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Toll-Like Receptors / metabolism
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Urticaria / blood
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Urticaria / genetics*
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Urticaria / metabolism
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Young Adult
Substances
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Cell Adhesion Molecules
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DC-specific ICAM-3 grabbing nonintegrin
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Lectins, C-Type
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Receptors, Cell Surface
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TLR7 protein, human
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Toll-Like Receptor 2
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Toll-Like Receptor 7
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Toll-Like Receptor 9
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Toll-Like Receptors