Structure of the type IX group B Streptococcus capsular polysaccharide and its evolutionary relationship with types V and VII

Abstract

The Group B Streptococcus capsular polysaccharide type IX was isolated and purified, and the structure of its repeating unit was determined. Type IX capsule → 4)[NeupNAc-α-(2 → 3)-Galp-β-(1 → 4)-GlcpNAc-β-(1 → 6)]-β-GlcpNAc-(1 → 4)-β-Galp-(1 → 4)-β-Glcp-(1 → appears most similar to types VII and V, although it contains two GlcpNAc residues. Genetic analysis identified differences in cpsM, cpsO, and cpsI gene sequences as responsible for the differentiation between the three capsular polysaccharide types, leading us to hypothesize that type V emerged from a recombination event in a type IX background.

Keywords: Bacterial Genetics; Capsular Polysaccharide; Group B Streptococcus; Nuclear Magnetic Resonance; Polysaccharide; Streptococcus; Vaccine.