DEPDC1B enhances migration and invasion of non-small cell lung cancer cells via activating Wnt/β-catenin signaling

Biochem Biophys Res Commun. 2014 Jul 18;450(1):899-905. doi: 10.1016/j.bbrc.2014.06.076. Epub 2014 Jun 24.

Abstract

Non-small cell lung cancer (NSCLC) remains a highly challenging and deadly malignancy with limited improvements in prognosis over years. Further understanding the molecular events involved in NSCLC oncogenesis and progression will help develop new and effective therapeutic strategies. In this study, we identified a ubiquitous up-regulation of DEPDC1B in NSCLC cell lines and clinical specimens, as well as its inverse correlation with patient survival. Ectopic expression of DEPDC1B endowed NSCLC cells with enhanced migration and invasion, while silencing its expression suppressed these traits. Mechanistic study showed that DEPDC1B was able to activate Wnt/β-catenin signaling, and that depletion of TCF4 or LEF1 abrogated the biological effects of DEPDC1B on cellular migration and invasion. Taken together, our data demonstrate that DEPDC1B might confer metastasis-related malignant phenotype to NSCLC in a Wnt/β-catenin dependent manner, providing new insights in developing novel anti-NSCLC strategies.

Keywords: DEPDC1B; Invasion; Migration; Non-small cell lung cancer; Wnt/β-catenin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Non-Small-Cell Lung / metabolism*
  • Carcinoma, Non-Small-Cell Lung / pathology*
  • Catenins / metabolism*
  • Cell Line, Tumor
  • Cell Movement
  • Humans
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / pathology*
  • Neoplasm Invasiveness
  • Signal Transduction
  • Wnt Proteins / metabolism*
  • Wnt Signaling Pathway*

Substances

  • Catenins
  • Wnt Proteins