Increasing the rate of muscle protein synthesis is an energy consuming process that explains the acute elevations in resting energy expenditure (REE) observed 12 to 72 hours after a resistance exercise session. We hypothesized that multiple sessions of resistance exercise combined with the intake of amino acids would increase REE and alter the nonprotein respiratory exchange ratio (RER). Ten male participants completed two separate seven-day trials where REE and RER were measured on each morning via indirect calorimetry. On four consecutive days within each seven-day trial, acute resistance exercise was performed, and nutritional intake was manipulated by providing (1) amino acids and carbohydrate (AA-RT) or (2) nonnitrogenous, isoenergetic carbohydrate (CHO-RT) before and during each resistance exercise session. Average REE within the training period was 3.61% greater in AA-RT (7897 ± 252 kJ) compared to CHO-RT (7622 ± 289 kJ; P = 0.02). RER declined (P < 0.0001) from baseline after each resistance exercise was initiated in both AA-RT (0.82 ± 0.01 to 0.77 ± 0.01) and CHO-RT (0.82 ± 0.02 to 0.77 ± 0.02). We conclude the provision of amino acids with multiple bouts of resistance exercise enhances energy expenditure at rest without altering the utilization of lipid.