Marinomonas profundimaris sp. nov., isolated from deep-sea sediment sample of the Arctic Ocean

Antonie Van Leeuwenhoek. 2014 Sep;106(3):449-55. doi: 10.1007/s10482-014-0213-5. Epub 2014 Jun 22.

Abstract

A taxonomic study was carried out on strain D104(T), which was isolated from deep-sea subsurface sediment sample from the Arctic Ocean. The bacterium was found to be Gram-negative, oxidase negative and catalase weakly positive, rod shaped, motile by means of polar flagellum. The organism grows between 4 and 37 °C (optimum 25-28 °C) and 0.5-6 % NaCl (optimum 3 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D104(T) belongs to the genus Marinomonas, with highest sequence similarities of 97.7 % to Marinomonas ushuaiensis DSM 15871(T), followed by M. dokdonensis DSW10-10(T) (96.9 %), M. arenicola KMM 3893(T) (96.7 %), M. arctica 328(T) (96.6 %) and other 18 species of the genus Marinomonas (94.4-96.5 %). The average nucleotide identity and estimated DNA-DNA hybridization values between strain D104(T) and M. ushuaiensis DSM 15871(T) were 84.24 % and 20.80 ± 2.33 % respectively. The principal fatty acids were C16:0, sum in feature 3 (C16:1 ω7c/C16:1 ω6c), sum in feature 8 (C18:1 ω7c/C18:1 ω6c) and C12:1 3OH. The G + C content of the chromosomal DNA was determined to be 44.8 mol%. The respiratory quinone was found to be Q8 (100 %). Polar lipids include phosphatidylglycerol and phosphatidylethanolamine as major phospholipids and aminolipid and phospholipid as minor components. The results of the genotypic and phenotypic analyses indicate that strain D104(T) represents a novel species of the genus Marinomonas, for which the name Marinomonas profundimaris sp. nov. is proposed, with the type strain D104(T) (=MCCC 1A07573(T) = LMG 27696(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arctic Regions
  • Bacterial Typing Techniques
  • Base Composition
  • Catalase / analysis
  • Cluster Analysis
  • Cytosol / chemistry
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Genome, Bacterial
  • Geologic Sediments / microbiology*
  • Locomotion
  • Marinomonas / classification*
  • Marinomonas / genetics
  • Marinomonas / isolation & purification*
  • Marinomonas / physiology
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oxidoreductases / analysis
  • Phospholipids / analysis
  • Phylogeny
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sodium Chloride / metabolism
  • Temperature

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Phospholipids
  • Quinones
  • RNA, Ribosomal, 16S
  • Sodium Chloride
  • Oxidoreductases
  • Catalase

Associated data

  • GENBANK/AYOZ00000000