Extended characterization of Corynebacterium pyruviciproducens based on clinical strains from Canada and Switzerland

J Clin Microbiol. 2014 Sep;52(9):3180-3. doi: 10.1128/JCM.00792-14. Epub 2014 Jun 20.

Abstract

The species Corynebacterium pyruviciproducens was described in 2010 based on the features of a single strain. In this report, we describe the chemotaxonomic and phenotypic characteristics of 11 C. pyruviciproducens clinical strains isolated in Switzerland and Canada in comparison to the strain 06-17730(T). Heterogeneities within the type strain were found in the 16S rRNA gene and in biochemical markers. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification of this species could not be achieved since currently this bacterial species is not included in the corresponding database. Reliable identification is obtained only with sequence-based identification tools. Results of antimicrobial susceptibility testing of this species with an extended panel of antimicrobials are presented here for the first time.

MeSH terms

  • Bacterial Typing Techniques
  • Canada
  • Cluster Analysis
  • Corynebacterium / classification*
  • Corynebacterium / genetics
  • Corynebacterium / isolation & purification*
  • Corynebacterium / physiology
  • Corynebacterium Infections / microbiology*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Switzerland

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/GU304654
  • GENBANK/GU304655
  • GENBANK/GU304656
  • GENBANK/GU304657
  • GENBANK/GU304658
  • GENBANK/GU304659
  • GENBANK/GU304660
  • GENBANK/JX501770
  • GENBANK/JX501771
  • GENBANK/JX501772
  • GENBANK/JX501773