A fluorescent light-up probe with AIE characteristics for specific mitochondrial imaging to identify differentiating brown adipose cells

Chem Commun (Camb). 2014 Aug 7;50(61):8312-5. doi: 10.1039/c4cc00452c.

Abstract

We report the design and synthesis of a specific mitochondrial fluorescent probe AIE-MitoGreen-1 with AIE characteristics to monitor the mitochondrial morphology changes and identify the differentiation process of living brown adipose cells. The probe AIE-MitoGreen-1 has significant advantages such as high cellpermeability, good mitochondrial retention, low background fluorescence, large Stokes shift, and low toxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / metabolism*
  • Cell Differentiation
  • Cell Survival / drug effects
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / toxicity
  • Humans
  • Hydrazines / chemistry*
  • Hydrazines / toxicity
  • Microscopy, Fluorescence
  • Mitochondria / pathology*
  • Pyridinium Compounds / chemistry*
  • Pyridinium Compounds / toxicity

Substances

  • AIE-MitoGreen-1
  • Fluorescent Dyes
  • Hydrazines
  • Pyridinium Compounds