Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice

Stem Cell Reports. 2014 Apr 24;2(5):662-74. doi: 10.1016/j.stemcr.2014.03.011. eCollection 2014 May 6.

Abstract

In this article, we show that mouse embryonic stem cell- or induced pluripotent stem cell-derived 3D retinal tissue developed a structured outer nuclear layer (ONL) with complete inner and outer segments even in an advanced retinal degeneration model (rd1) that lacked ONL. We also observed host-graft synaptic connections by immunohistochemistry. This study provides a "proof of concept" for retinal sheet transplantation therapy for advanced retinal degenerative diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Collagen / chemistry
  • Drug Combinations
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / transplantation*
  • Immunohistochemistry
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / transplantation*
  • Laminin / chemistry
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mitochondria / metabolism
  • Mitochondria / pathology
  • Proteoglycans / chemistry
  • Receptors, Retinoic Acid / antagonists & inhibitors
  • Retina / metabolism
  • Retina / pathology*
  • Retinal Degeneration / therapy*
  • Retinal Degeneration / veterinary
  • Transplantation, Homologous

Substances

  • Drug Combinations
  • Laminin
  • Proteoglycans
  • Receptors, Retinoic Acid
  • matrigel
  • Collagen