Formation of newly synthesized adeno-associated virus capsids in the cell nucleus

Hum Gene Ther Methods. 2014 Jun;25(3):179-80. doi: 10.1089/hgtb.2014.054.

Abstract

Adeno-associated virus (AAV) particles inside the nucleus of a HEK 293 cell are shown by electron microscopy. Cells have been triple-transfected for vector production and were analyzed for capsid formation three days later. Newly assembled particle are visible as seemingly unstructured conglomerates or crystal-like arrays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid Proteins / genetics
  • Capsid Proteins / metabolism*
  • Cell Nucleus / metabolism*
  • Cell Nucleus / virology*
  • Dependovirus / metabolism*
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • HEK293 Cells
  • Humans
  • Microscopy, Electron

Substances

  • Capsid Proteins