Analysis of species and intra-species associations between the Mycobacterium abscessus complex strains using pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST)

Se-Mi Jeon; Na-Ra Lim; Seung-Jik Kwon; Tae-Sun Shim; Mi-Sun Park; Bum-Joon Kim; Seong-Han Kim

doi:10.1016/j.mimet.2014.05.024

Analysis of species and intra-species associations between the Mycobacterium abscessus complex strains using pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST)

J Microbiol Methods. 2014 Sep:104:19-25. doi: 10.1016/j.mimet.2014.05.024. Epub 2014 Jun 8.

Authors

Se-Mi Jeon¹, Na-Ra Lim¹, Seung-Jik Kwon¹, Tae-Sun Shim², Mi-Sun Park¹, Bum-Joon Kim³, Seong-Han Kim⁴

Affiliations

¹ Division of Tuberculosis and Bacterial Respiratory Infections, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Chungbuk, Republic of Korea.
² Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Republic of Korea.
³ Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute, Cancer Research Institute and Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address: kbumjoon@snu.ac.kr.
⁴ Division of Tuberculosis and Bacterial Respiratory Infections, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Chungbuk, Republic of Korea. Electronic address: kkingsh@chol.com.

PMID: 24918987
DOI: 10.1016/j.mimet.2014.05.024

Abstract

PFGE and MLST showed that the strains of M. massiliense hsp65 II-1 were clearly separated from the strains of M. massiliense hsp65 I or II-2 as well as the strains of M. abscessus or M. bolletii; thus, M. massiliense hsp6 5II-1 might represent an additional subspecies of M. massiliense.

Keywords: M. abscessus complex; M. bolletii; M. massiliense; MLST; PFGE.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Typing Techniques / methods*
Electrophoresis, Gel, Pulsed-Field / methods*
Humans
Multilocus Sequence Typing / methods*
Mycobacterium / classification*
Mycobacterium / drug effects
Mycobacterium / genetics
Mycobacterium / isolation & purification*
Mycobacterium Infections / microbiology
Phylogeny

Substances

Anti-Bacterial Agents
Bacterial Proteins