Microbiological evaluation of the efficacy of soapy water to clean hands: a randomized, non-inferiority field trial

Am J Trop Med Hyg. 2014 Aug;91(2):415-23. doi: 10.4269/ajtmh.13-0475. Epub 2014 Jun 9.

Abstract

We conducted a randomized, non-inferiority field trial in urban Dhaka, Bangladesh among mothers to compare microbial efficacy of soapy water (30 g powdered detergent in 1.5 L water) with bar soap and water alone. Fieldworkers collected hand rinse samples before and after the following washing regimens: scrubbing with soapy water for 15 and 30 seconds; scrubbing with bar soap for 15 and 30 seconds; and scrubbing with water alone for 15 seconds. Soapy water and bar soap removed thermotolerant coliforms similarly after washing for 15 seconds (mean log10 reduction = 0.7 colony-forming units [CFU], P < 0.001 for soapy water; mean log10 reduction = 0.6 CFU, P = 0.001 for bar soap). Increasing scrubbing time to 30 seconds did not improve removal (P > 0.05). Scrubbing hands with water alone also reduced thermotolerant coliforms (mean log10 reduction = 0.3 CFU, P = 0.046) but was less efficacious than scrubbing hands with soapy water. Soapy water is an inexpensive and microbiologically effective cleansing agent to improve handwashing among households with vulnerable children.

Publication types

  • Randomized Controlled Trial
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bangladesh
  • Child, Preschool
  • Clostridium perfringens / drug effects*
  • Colony Count, Microbial
  • Developing Countries
  • Escherichia coli / drug effects*
  • Female
  • Hand Disinfection / methods*
  • Hot Temperature
  • Humans
  • Microbial Viability / drug effects
  • Mothers
  • Soaps / pharmacology*
  • Water

Substances

  • Soaps
  • Water