A novel quinoline molecular probe and the derived functionalized gold nanoparticles: sensing properties and cytotoxicity studies in MCF-7 human breast cancer cells

Cristina Núñez; Elisabete Oliveira; Javier García-Pardo; Mario Diniz; Julia Lorenzo; José Luis Capelo; Carlos Lodeiro

doi:10.1016/j.jinorgbio.2014.04.007

A novel quinoline molecular probe and the derived functionalized gold nanoparticles: sensing properties and cytotoxicity studies in MCF-7 human breast cancer cells

J Inorg Biochem. 2014 Aug:137:115-22. doi: 10.1016/j.jinorgbio.2014.04.007. Epub 2014 Apr 24.

Authors

Cristina Núñez¹, Elisabete Oliveira², Javier García-Pardo³, Mario Diniz⁴, Julia Lorenzo⁵, José Luis Capelo⁶, Carlos Lodeiro⁷

Affiliations

¹ REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte da Caparica, Portugal; Ecology Research Group, Department of Geographical and Life Sciences, Canterbury Christ Church University, CT1 1QU Canterbury, United Kingdom; Inorganic Chemistry Department, Faculty of Chemistry, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. Electronic address: cristina.nunez@fct.unl.pt.
² REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte da Caparica, Portugal; ProteoMass Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal; Veterinary Science Department, (CECAV), University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal.
³ Institut de Biotecnologia i de Biomedicina and Departament de Bioquímica i de Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
⁴ REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte da Caparica, Portugal.
⁵ Institut de Biotecnologia i de Biomedicina and Departament de Bioquímica i de Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain. Electronic address: julia.lorenzo@uab.es.
⁶ REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte da Caparica, Portugal; ProteoMass Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal.
⁷ REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte da Caparica, Portugal; ProteoMass Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal. Electronic address: cle@fct.unl.pt.

PMID: 24861645
DOI: 10.1016/j.jinorgbio.2014.04.007

Abstract

A highly selective quinoline-based fluorescent sensor L was designed, prepared and used to monitor zinc ions in Goldfish (Carassius auratus) as model of vertebrate organism. Modified gold nanoparticles having functional quinoline molecules (GNPs@L) were also synthesized and their sensing properties towards different metal ions were also explored in solution, showing high selectively towards the toxic and heavy metal ion mercury. Cell proliferation kit XTT that employs 2,3-bis-(2-methoxy-4-nitro- 5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT) was used in order to investigate the cytotoxicity of compound L and GNPs@L on the MCF-7 breast cancer cells, showing significant cytotoxicity in comparison with similar reported systems. It was observed that L and GNPs@L compounds induced apoptosis in MCF-7 cancer cells. The cellular uptake of the hybrid system GNPs@L was studied using confocal laser scanning microscopy (CLSM).

Keywords: Cytotoxicity; Fluorescent probes; Nanoparticles; Quinoline; XTT assay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breast Neoplasms / drug therapy*
Breast Neoplasms / metabolism
Breast Neoplasms / pathology
Cell Proliferation / drug effects
Female
Gold / administration & dosage
Gold / chemistry*
Humans
MCF-7 Cells
Metal Nanoparticles / administration & dosage
Metal Nanoparticles / chemistry*
Quinolines / administration & dosage
Quinolines / chemistry*

Substances

Quinolines
Gold
quinoline