Phosphoseryl-tRNA in Escherichia coli

Nucleic Acids Symp Ser. 1989:(21):25-6.

Abstract

Two seryl-tRNAs, which were prepared from natural suppressor tRNA-rich fractions on a pattern of chromatography on Sephadex A-50, were phosphorylated by a tRNA kinase in Escherichia coli B. A part of phosphate on the tRNA was confirmed to be phosphoserine. Phosphoseryl-tRNA is universal in bacteria and vertebrates. Phosphoseryl-tRNA should be an intermediate from seryl-tRNA to selenocysteyl-tRNA.

MeSH terms

  • Chromatography, Gel
  • Escherichia coli / genetics*
  • Nucleic Acid Hybridization
  • Phosphoserine / analysis*
  • RNA, Bacterial / analysis*
  • RNA, Transfer, Amino Acid-Specific / analysis*
  • RNA, Transfer, Ser / analysis*
  • Serine / analogs & derivatives*

Substances

  • RNA, Bacterial
  • RNA, Transfer, Amino Acid-Specific
  • RNA, Transfer, Ser
  • Phosphoserine
  • Serine