A new method for rapid screening of ester-producing yeasts using in situ HS-SPME

Juliano Garavaglia; Andressa Habekost; Thiago Rodrigues Bjerk; Rosana de Cassia de Souza Schneider; Juliane Elisa Welke; Cláudia Alcaraz Zini; Patricia Valente

doi:10.1016/j.mimet.2014.05.001

A new method for rapid screening of ester-producing yeasts using in situ HS-SPME

J Microbiol Methods. 2014 Aug:103:1-2. doi: 10.1016/j.mimet.2014.05.001. Epub 2014 May 9.

Authors

Juliano Garavaglia¹, Andressa Habekost², Thiago Rodrigues Bjerk², Rosana de Cassia de Souza Schneider³, Juliane Elisa Welke⁴, Cláudia Alcaraz Zini⁵, Patricia Valente⁶

Affiliations

¹ Federal University of Health Sciences of Porto Alegre, Av. Sarmento Leite, 245, office 208, 90050-170, Porto Alegre, RS, Brazil. Electronic address: julianogr@ufcspa.edu.br.
² Chromatography Laboratory, University of Santa Cruz do Sul, Av. Independência, 2293, 96815-900, Santa Cruz do Sul, RS, Brazil.
³ Chromatography Laboratory, University of Santa Cruz do Sul, Av. Independência, 2293, 96815-900, Santa Cruz do Sul, RS, Brazil. Electronic address: rosana@unisc.br.
⁴ Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, office 118, 91.501-970, Porto Alegre, RS, Brazil. Electronic address: juliane.welke@ufrgs.br.
⁵ Institute of Chemistry, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, office 210, 91.501-970, Porto Alegre, RS, Brazil. Electronic address: cazini@iq.ufrgs.br.
⁶ Department of Microbiology, Immunology and Parasitology, Federal University of Rio Grande do Sul, Av. Sarmento Leite, 500, office 154, 90050-170, Porto Alegre, RS, Brazil. Electronic address: patricia.valente@ufrgs.br.

PMID: 24814753
DOI: 10.1016/j.mimet.2014.05.001

Abstract

The selection of ester-producing yeasts is difficult because these molecules evaporate quickly, are extremely unstable and may be missed during analytical manipulation. We propose an easy, fast and efficient headspace-SPME method for screening of ester-producing yeasts directly at the extraction vials (in situ HS-SPME).

Keywords: Esters; Gas chromatography; Mass spectrometer; Screening; Yeast fermentation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Esters / chemistry
Esters / metabolism*
Gas Chromatography-Mass Spectrometry*
Solid Phase Microextraction / methods*
Yeasts / metabolism*

Substances

Esters