Electrophysiological expression of endothelin and angiotensin receptors in Xenopus oocytes injected with rat heart mRNA

FEBS Lett. 1989 Dec 4;258(2):289-92. doi: 10.1016/0014-5793(89)81676-x.

Abstract

Functional endothelin and angiotensin receptors have been expressed in Xenopus oocyte following the microinjection of rat heart mRNA. Under voltage clamp conditions, application of these peptides clearly induced oscillatory Ca2+-activated chloride currents in a dose-dependent manner. In addition, no direct modulation of expressed or native cardiac Ca channels was observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin II / pharmacology*
  • Animals
  • Chloride Channels
  • Chlorides / physiology*
  • Electric Conductivity
  • Endothelins
  • Endothelium, Vascular / physiology
  • Female
  • Gene Expression*
  • Heart Ventricles / drug effects
  • Ion Channels / drug effects
  • Ion Channels / physiology*
  • Kinetics
  • Membrane Proteins / physiology*
  • Microinjections
  • Myocardium / metabolism*
  • Oocytes / drug effects
  • Oocytes / metabolism
  • Oocytes / physiology*
  • Peptides / pharmacology*
  • RNA, Messenger / genetics*
  • Rats
  • Receptors, Angiotensin / genetics*
  • Receptors, Angiotensin / physiology
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / physiology
  • Receptors, Endothelin
  • Ventricular Function
  • Xenopus

Substances

  • Chloride Channels
  • Chlorides
  • Endothelins
  • Ion Channels
  • Membrane Proteins
  • Peptides
  • RNA, Messenger
  • Receptors, Angiotensin
  • Receptors, Cell Surface
  • Receptors, Endothelin
  • Angiotensin II