Sample treatment platform using nanoparticles to determine salinomycin in flesh and meat

Jihyun Park; H B Lim

doi:10.1016/j.foodchem.2014.03.047

Sample treatment platform using nanoparticles to determine salinomycin in flesh and meat

Food Chem. 2014 Oct 1:160:112-7. doi: 10.1016/j.foodchem.2014.03.047. Epub 2014 Mar 18.

Authors

Jihyun Park¹, H B Lim²

Affiliations

¹ Department of Chemistry, NSBI, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701, Republic of Korea.
² Department of Chemistry, NSBI, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701, Republic of Korea. Electronic address: plasma@dankook.ac.kr.

PMID: 24799216
DOI: 10.1016/j.foodchem.2014.03.047

Abstract

In this work, we developed a sensitive and selective analytical method of determining salinomycin in flesh and meat using a lab-built laser induced fluorescence microscope (LIFM) with nanoparticles. Two types of nanoparticles, Cy5 doped core-shell silica nanoparticles as a probe and magnetic nanoparticles (MNPs) to extract the target antibiotic from the treated sample, were synthesized and modified for chemical and enzymatic binding. After optimization, the newly developed method was applied to ham, chicken, and meat samples for the quantitative determination of salinomycin. The limits of detection (LODs) obtained from the calibration curve were 48-590 pg/mL, which was about 100-fold lower than that of the ELISA method.

Keywords: Cy5-doped silica nanoparticles; Laser-induced fluorescence; Salinomycin; Sample treatment platform.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / analysis*
Limit of Detection
Meat / analysis*
Microscopy, Fluorescence
Nanoparticles*
Pyrans / analysis*
Silicon Dioxide

Substances

Anti-Bacterial Agents
Pyrans
salinomycin
Silicon Dioxide