Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II

Nature. 1989 Oct 5;341(6241):410-4. doi: 10.1038/341410a0.

Abstract

RAP30/74 is a heteromeric general transcription initiation factor which binds to RNA polymerase II. Here we report that preparations of RAP30/74 contain an ATP-dependent DNA helicase whose probable function is to melt the DNA at transcriptional start sites. The sequence of the RAP30 subunit of RAP30/74 indicates that RAP30 may be distantly related to bacterial sigma factors.

MeSH terms

  • Amino Acid Sequence
  • Bacillus subtilis / genetics
  • Base Sequence
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cloning, Molecular
  • DNA Helicases / metabolism*
  • Escherichia coli / genetics
  • Genes*
  • Humans
  • Kinetics
  • Macromolecular Substances
  • Molecular Sequence Data
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism
  • Protein Binding
  • RNA Polymerase II / metabolism*
  • Sarcosine / pharmacology
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • Carrier Proteins
  • Macromolecular Substances
  • Phosphoproteins
  • RNA polymerase II-binding proteins
  • sarkosyl
  • RNA Polymerase II
  • DNA Helicases
  • Sarcosine