Isolation and sequence of lambda gt11 cDNA clones encoding the 5B4 antigen expressed on sprouting neurons

Brain Res Mol Brain Res. 1989 Jun;5(4):297-303. doi: 10.1016/0169-328x(89)90064-8.

Abstract

Monoclonal antibody (mAb) 5B4 recognizes a developmentally regulated membrane glycoprotein (Mr approximately 185,000-255,000) expressed on sprouting neurons. The amino acid sequence deduced from lambda gt11 cDNA clones encoding the transmembrane and cytoplasmic domains of the 5B4 antigen is co-linear with that of chick NCAM-ld. The significant level of overall sequence identity (75%) demonstrates that the 5B4 antigen is rat brain NCAM. The 5B4 epitope maps to the carboxy-terminus common to both NCAM-ld and NCAM-sd.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal*
  • Antigens, Surface / analysis*
  • Base Sequence
  • Cell Adhesion Molecules
  • Dendrites / metabolism*
  • Epitopes
  • Membrane Glycoproteins / analysis*
  • Molecular Sequence Data
  • Molecular Weight

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Cell Adhesion Molecules
  • Epitopes
  • Membrane Glycoproteins