Abstract
Validation of a mouse model of dengue virus (DENV) infection relies on verification of viremia and productive replication in mouse tissues following infection. Here, we describe a quantitative assay for determining viral RNA levels in mouse serum and tissues. For the purpose of confirming DENV replication, we outline a fluorescence immunohistochemistry (FIHC) protocol for staining a nonstructural protein of DENV.
MeSH terms
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Animals
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Dengue / virology*
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Dengue Virus / genetics
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Disease Models, Animal*
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Fluorescence
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Immunohistochemistry
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Mice
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RNA / isolation & purification
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RNA Helicases / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Serine Endopeptidases / metabolism
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Spleen / virology
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Viral Nonstructural Proteins / metabolism
Substances
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NS3 protein, flavivirus
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Viral Nonstructural Proteins
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RNA
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Serine Endopeptidases
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RNA Helicases