An evaluation of multiple phenotypic screening methods for Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae

J Infect Chemother. 2014 Mar;20(3):224-7. doi: 10.1016/j.jiac.2013.10.011. Epub 2013 Dec 11.

Abstract

Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae may display MICs to carbapenems within susceptible or intermediate ranges, prompting confirmatory testing. Four phenotypic methods to detect KPC producers were evaluated against a collection of clinical Enterobacteriaceae isolates. Meropenem-phenylboronic acid double disk synergy testing demonstrated the best performance with 100% sensitivity and specificity.

Keywords: CRE; Carbapenem; Chromogenic agar; Modified Hodge test; Phenylboronic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / biosynthesis*
  • Bacterial Typing Techniques / methods*
  • Boronic Acids
  • Humans
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / classification*
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics
  • Microbial Sensitivity Tests
  • Phenotype
  • Sensitivity and Specificity
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / biosynthesis*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Boronic Acids
  • beta-Lactamases
  • carbapenemase
  • benzeneboronic acid