Self-immolative bioluminogenic quinone luciferins for NAD(P)H assays and reducing capacity-based cell viability assays

Chembiochem. 2014 Mar 21;15(5):670-5. doi: 10.1002/cbic.201300744. Epub 2014 Mar 3.

Abstract

Highly sensitive self-cleavable trimethyl lock quinone-luciferin substrates for diaphorase were designed and synthesized to measure NAD(P)H in biological samples and monitor viable cells via NAD(P)H-dependent cellular oxidoreductase enzymes and their NAD(P)H cofactors.

Keywords: NAD(P)H; diaphorase; quinones; reducing capacity; self-immolative linkers.

MeSH terms

  • Cell Line, Tumor
  • Cell Survival
  • Firefly Luciferin / analogs & derivatives*
  • Firefly Luciferin / analysis
  • Firefly Luciferin / metabolism
  • Humans
  • Luminescent Agents / analysis
  • Luminescent Agents / metabolism*
  • Luminescent Measurements
  • NADP / analysis
  • NADP / metabolism*
  • Quinones / analysis
  • Quinones / metabolism*

Substances

  • Luminescent Agents
  • Quinones
  • NADP
  • Firefly Luciferin