Fast, multi-dimensional and simultaneous kymograph-like particle dynamics (SkyPad) analysis

Bruno Cadot; Vincent Gache; Edgar R Gomes

doi:10.1371/journal.pone.0089073

Fast, multi-dimensional and simultaneous kymograph-like particle dynamics (SkyPad) analysis

PLoS One. 2014 Feb 19;9(2):e89073. doi: 10.1371/journal.pone.0089073. eCollection 2014.

Authors

Bruno Cadot¹, Vincent Gache², Edgar R Gomes¹

Affiliations

¹ UMRS 787 INSERM, Université Pierre et Marie Curie Paris 6, Paris, France ; Groupe Hospitalier Pitié-Salpêtrière, Institut de Myologie, Paris, France.
² UMRS 787 INSERM, Université Pierre et Marie Curie Paris 6, Paris, France.

Abstract

Background: Kymograph analysis is a method widely used by researchers to analyze particle dynamics in one dimensional (1D) trajectories.

Results: Here we provide a Visual Basic-coded algorithm to use as a Microsoft Excel add-in that automatically analyzes particles in 2D trajectories with all the advantages of kymograph analysis.

Conclusions: This add-in, which we named SkyPad, leads to significant time saving and higher accuracy of particle analysis. Finally, SkyPad can also be used for 3D trajectories analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms*
Animals
Cells, Cultured
Imaging, Three-Dimensional / methods*
Kinetics
Kymography / methods*
Mice
Software*
User-Computer Interface

Grants and funding

This work was supported by Muscular Dystrophy Association (MDA), INSERM Avenir programme and Agence Nationale de la Recherche (ANR) grants to E.R.G. B.C. was supported initially by a Fondation pour la Recherche Médicale (FRM) fellowship. V.G. was supported initially by a Region Ile-de-France fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.