Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

Kuangwen Hsieh; Peter L Mage; Andrew T Csordas; Michael Eisenstein; H Tom Soh

doi:10.1039/c4cc00540f

Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

Chem Commun (Camb). 2014 Apr 11;50(28):3747-9. doi: 10.1039/c4cc00540f. Epub 2014 Feb 28.

Authors

Kuangwen Hsieh¹, Peter L Mage, Andrew T Csordas, Michael Eisenstein, H Tom Soh

Affiliation

¹ Department of Mechanical Engineering, University of California, Santa Barbara, CA 93106, USA. tsoh@engr.ucsb.edu.

PMID: 24577617
DOI: 10.1039/c4cc00540f

Abstract

We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.

MeSH terms

DNA, Bacterial / analysis*
DNA, Bacterial / chemistry
Nucleic Acid Amplification Techniques / methods*
Salmonella typhimurium / genetics
Uracil-DNA Glycosidase / chemistry

Substances

DNA, Bacterial
Uracil-DNA Glycosidase