Antiphosphotyrosine recovery of phospholipase C activity after EGF treatment of A-431 cells

Science. 1988 Aug 19;241(4868):968-70. doi: 10.1126/science.2457254.

Abstract

A tenfold increase in phospholipase C activity specific for phosphatidylinositol 4,5-bisphosphate (PIP2) was immunopurified from extracts of A-431 epidermoid carcinoma cells stimulated with epidermal growth factor. This finding suggests a biochemical link between growth factor-stimulated tyrosine kinase activity and PIP2 hydrolysis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies / immunology
  • Carcinoma, Squamous Cell
  • Chromatography, High Pressure Liquid
  • Cytosol / enzymology
  • Epidermal Growth Factor / pharmacology*
  • Hydrolysis
  • Inositol Phosphates / metabolism
  • Phosphatidylinositol 4,5-Diphosphate
  • Phosphatidylinositols / metabolism
  • Phosphorylation
  • Phosphotyrosine
  • Protein-Tyrosine Kinases / metabolism
  • Substrate Specificity
  • Tumor Cells, Cultured
  • Type C Phospholipases / metabolism*
  • Tyrosine / analogs & derivatives*
  • Tyrosine / immunology
  • Tyrosine / metabolism

Substances

  • Antibodies
  • Inositol Phosphates
  • Phosphatidylinositol 4,5-Diphosphate
  • Phosphatidylinositols
  • Phosphotyrosine
  • Tyrosine
  • Epidermal Growth Factor
  • Protein-Tyrosine Kinases
  • Type C Phospholipases