Knockdown of 14-3-3ζ enhances radiosensitivity and radio-induced apoptosis in CD133(+) liver cancer stem cells

Exp Mol Med. 2014 Feb 21;46(2):e77. doi: 10.1038/emm.2013.151.

Abstract

14-3-3ζ is related to many cancer survival cellular processes. In a previous study, we showed that silencing 14-3-3ζ decreases the resistance of hepatocellular carcinoma (HCC) to chemotherapy. In this study, we investigated whether silencing 14-3-3ζ affects the radioresistance of cancer stem-like cells (CSCs) in HCC. Knockdown of 14-3-3ζ decreased cell viability and the number of spheres by reducing radioresistance in CSCs after γ-irradiation (IR). Furthermore, the levels of pro-apoptotic proteins were upregulated in CSCs via silencing 14-3-3ζ after IR. These results suggest that 14-3-3ζ knockdown enhances radio-induced apoptosis by reducing radioresistance in liver CSCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins / genetics
  • 14-3-3 Proteins / metabolism*
  • AC133 Antigen
  • Antigens, CD / genetics
  • Antigens, CD / metabolism*
  • Apoptosis Regulatory Proteins / genetics
  • Apoptosis Regulatory Proteins / metabolism
  • Carcinoma, Hepatocellular / genetics
  • Carcinoma, Hepatocellular / metabolism
  • Cell Line, Tumor
  • Gamma Rays*
  • Glycoproteins / genetics
  • Glycoproteins / metabolism*
  • Humans
  • Liver Neoplasms / genetics
  • Liver Neoplasms / metabolism
  • Neoplastic Stem Cells / metabolism
  • Neoplastic Stem Cells / radiation effects*
  • Peptides / genetics
  • Peptides / metabolism*
  • Radiation Tolerance*

Substances

  • 14-3-3 Proteins
  • AC133 Antigen
  • Antigens, CD
  • Apoptosis Regulatory Proteins
  • Glycoproteins
  • PROM1 protein, human
  • Peptides