Comparison of a transplant multiplex viral panel on the ICEPlex system with real-time PCR for detection of cytomegalovirus, Epstein-Barr virus, and BK virus in clinical specimens

J Clin Microbiol. 2014 May;52(5):1711-3. doi: 10.1128/JCM.00358-14. Epub 2014 Feb 19.

Abstract

We compared a multiplex viral transplant panel on the ICEPlex system to real-time PCR for the detection of cytomegalovirus (CMV), Epstein-Barr virus (EBV), and BK virus (BKV). The sensitivities of the ICEPlex were 83.3%, 95.5%, and 65.5% for the detection of CMV, EBV, and BKV, respectively. Interestingly, the multiplex assay detected dual infections in 16/280 (5.7%) samples tested.

MeSH terms

  • BK Virus / genetics*
  • Cytomegalovirus / genetics*
  • Cytomegalovirus Infections / diagnosis
  • Cytomegalovirus Infections / virology
  • DNA, Viral / genetics
  • Epstein-Barr Virus Infections / diagnosis
  • Epstein-Barr Virus Infections / virology
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Polyomavirus Infections / diagnosis
  • Polyomavirus Infections / virology
  • Real-Time Polymerase Chain Reaction / methods*
  • Tumor Virus Infections / diagnosis

Substances

  • DNA, Viral