The pre-mRNA splicing reaction

Somsakul Pop Wongpalee; Shalini Sharma

doi:10.1007/978-1-62703-980-2_1

The pre-mRNA splicing reaction

Methods Mol Biol. 2014:1126:3-12. doi: 10.1007/978-1-62703-980-2_1.

Authors

Somsakul Pop Wongpalee¹, Shalini Sharma

Affiliation

¹ Department of Immunology and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles, CA, USA.

PMID: 24549652
DOI: 10.1007/978-1-62703-980-2_1

Abstract

In eukaryotic organisms, nascent transcripts of protein-coding genes contain intronic sequences that are not present in mature mRNAs. Pre-mRNA splicing removes introns and joins exons to form mature mRNAs. It is catalyzed by a large RNP complex called the spliceosome. Sequences within the pre-mRNA determine intron recognition and excision. This process occurs with a high degree of accuracy to generate the functional transcriptome of a cell.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Base Sequence
Exons / genetics
Gene Expression Profiling
Humans
Introns / genetics
Molecular Biology / methods*
RNA Precursors / genetics*
RNA Splicing*
Spliceosomes / genetics*

Substances

RNA Precursors

Grants and funding

CA170786/CA/NCI NIH HHS/United States