Antihyperglycemic, hypolipidemic, hepatoprotective and antioxidative effects of dietary clove (Szyzgium aromaticum) bud powder in a high-fat diet/streptozotocin-induced diabetes rat model

J Sci Food Agric. 2014 Oct;94(13):2726-37. doi: 10.1002/jsfa.6617. Epub 2014 Apr 14.

Abstract

Background: Syzygium aromaticum (L.) Merr. & Perry (clove) bud is an important spice used in the preparation of several delicacies and in folklore for diabetes management. The present study was convened to assess the effects of dietary clove bud powder (CBP) on biochemical parameters in a type 2 diabetes rat model, induced by a combination of high-fat diet and low-dose streptozotocin (35 mg kg⁻¹) for 30 days.

Results: Diabetic rats were placed on dietary regimen containing 20-40 g kg⁻¹ clove bud powder. The results revealed that there was no significant (P > 0.05) difference in the average feed intake and weight changes between the rat groups. Furthermore, supplementation with CBP gradually reduced blood glucose level in diabetic rat compared to control diabetic rats without CBP supplementation (DBC). Moreover, reduced activity of α-glucosidase was observed in CBP and metformin-treated rat groups when compared to that of the DBC rat group. In addition, the DBC group had significantly (P < 0.05) higher lipid concentrations (except for high-density lipoprotein cholesterol) when compared to all other groups. Furthermore, CBP had significantly (P < 0.05) reduced activity of liver enzymes (alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase) and showed elevated levels of antioxidant status (glutathione, ascorbic acid, superoxide dismutase and catalase).

Conclusion: The results suggest that the clove bud diet may attenuate hyperglycemia, hyperlipidemia, hepatotoxicity and oxidative stress in the type 2 diabetic condition.

Keywords: clove bud powder; high-fat diet; streptozotocin; type 2 diabetes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / adverse effects
  • Antioxidants / therapeutic use
  • Biomarkers / metabolism
  • Diabetes Mellitus, Type 2 / complications
  • Diabetes Mellitus, Type 2 / diet therapy*
  • Diabetes Mellitus, Type 2 / metabolism
  • Diabetes Mellitus, Type 2 / physiopathology
  • Diet, High-Fat / adverse effects
  • Dietary Supplements* / adverse effects
  • Ethnopharmacology
  • Flowers / chemistry
  • Flowers / growth & development
  • Hypoglycemic Agents / administration & dosage
  • Hypoglycemic Agents / adverse effects
  • Hypoglycemic Agents / therapeutic use*
  • Hypolipidemic Agents / administration & dosage
  • Hypolipidemic Agents / adverse effects
  • Hypolipidemic Agents / therapeutic use*
  • Lipotropic Agents / administration & dosage
  • Lipotropic Agents / adverse effects
  • Lipotropic Agents / therapeutic use
  • Liver / enzymology
  • Liver / metabolism
  • Liver / physiopathology
  • Male
  • Medicine, African Traditional
  • Nigeria
  • Non-alcoholic Fatty Liver Disease / complications
  • Non-alcoholic Fatty Liver Disease / diet therapy*
  • Non-alcoholic Fatty Liver Disease / metabolism
  • Non-alcoholic Fatty Liver Disease / physiopathology
  • Oxidative Stress
  • Phytotherapy / adverse effects
  • Plant Preparations / administration & dosage
  • Plant Preparations / adverse effects
  • Plant Preparations / therapeutic use*
  • Random Allocation
  • Rats, Wistar
  • Syzygium / chemistry*
  • Syzygium / growth & development

Substances

  • Antioxidants
  • Biomarkers
  • Hypoglycemic Agents
  • Hypolipidemic Agents
  • Lipotropic Agents
  • Plant Preparations