Cloning of genomic fragment from Plasmodium chabaudi expressing a 105 kilodalton antigen epitope

Mol Biochem Parasitol. 1988 Mar;28(2):153-61. doi: 10.1016/0166-6851(88)90063-1.

Abstract

The 105 kDa antigen of Plasmodium chabaudi has many characteristics of the P. falciparum Pf 155 (RESA) molecule. A clone (pPC105e) from a P. chabaudi genomic library was isolated using immune screening with a 105 kDa antigen specific monoclonal antibody (B7E10). Southern, Northern and Western blotting analyses provide evidence for a lack of variability at the protein and DNA levels. A subclone of the insert in the expression vector pEX2, synthesises a fusion peptide which contains the epitope recognized by B7E10. Sequences homologous to the insert were detected in the genome of three other rodent and two primate malarias.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Protozoan / genetics*
  • Cloning, Molecular
  • DNA / genetics
  • DNA Restriction Enzymes
  • Deoxyribonuclease EcoRI
  • Electrophoresis, Agar Gel
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes / genetics
  • Immunoassay
  • Nucleic Acid Hybridization
  • Plasmodium / genetics*
  • Plasmodium / immunology
  • RNA / genetics
  • Recombinant Fusion Proteins / analysis

Substances

  • Antigens, Protozoan
  • Epitopes
  • Recombinant Fusion Proteins
  • RNA
  • DNA
  • DNA Restriction Enzymes
  • Deoxyribonuclease EcoRI