Genotyping species of the Sporothrix schenckii complex by PCR-RFLP of calmodulin

Anderson Messias Rodrigues; G Sybren de Hoog; Zoilo Pires de Camargo

doi:10.1016/j.diagmicrobio.2014.01.004

Genotyping species of the Sporothrix schenckii complex by PCR-RFLP of calmodulin

Diagn Microbiol Infect Dis. 2014 Apr;78(4):383-7. doi: 10.1016/j.diagmicrobio.2014.01.004. Epub 2014 Jan 14.

Authors

Anderson Messias Rodrigues¹, G Sybren de Hoog², Zoilo Pires de Camargo³

Affiliations

¹ Department of Microbiology, Immunology and Parasitology, Cellular Biology Division, Federal University of São Paulo, São Paulo, Brazil; CBS-KNAW Fungal Biodiversity Center, Utrecht, The Netherlands.
² CBS-KNAW Fungal Biodiversity Center, Utrecht, The Netherlands.
³ Department of Microbiology, Immunology and Parasitology, Cellular Biology Division, Federal University of São Paulo, São Paulo, Brazil. Electronic address: zpcamargo@unifesp.br.

PMID: 24525143
DOI: 10.1016/j.diagmicrobio.2014.01.004

Abstract

Sporotrichosis is one of the most common subcutaneous mycosis in Latin America and is caused by 4 pathogenic thermodimorphic fungi in the genus Sporothrix. From both therapeutic and epidemiological perspectives, it is essential to identify the causative agents down to the species level. Traditional parameters may overlap among closely related species, and we propose polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as an alternative approach. In the present study, the calmodulin gene was amplified and digested with HhaI to yield 5 different electrophoretic patterns representing all medically important Sporothrix species: Sporothrix brasiliensis, Sporothrix schenckii sensu stricto, Sporothrix globosa, and Sporothrix luriei. The PCR-RFLP protocol described here is a simple and inexpensive method and is highly suitable for accurate routine genotyping of relevant Sporothrix species.

Keywords: Epidemiology; Molecular identification; PCR-RFLP; Sporothrix brasiliensis; Sporothrix schenckii; Sporotrichosis.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Calmodulin / genetics*
Humans
Latin America
Molecular Typing / methods*
Mycological Typing Techniques / methods*
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length*
Sporothrix / classification*
Sporothrix / genetics
Sporotrichosis / microbiology*

Substances

Calmodulin