(1)H, (13)C, and (15)N backbone and side-chain chemical shift assignments for the 36 proline-containing, full length 29 kDa human chimera-type galectin-3

Biomol NMR Assign. 2015 Apr;9(1):59-63. doi: 10.1007/s12104-014-9545-3. Epub 2014 Feb 7.

Abstract

Galectin-3, an adhesion/growth regulatory lectin, has a unique trimodular design consisting of the canonical carbohydrate recognition domain, a collagen-like tandem-repeat section, and an N-terminal peptide with two sites for Ser phosphorylation. Structural characterization of the full length protein with its non-lectin part (115 of 250 residues total) will help understand the multi functionality of this potent cellular effector. Here, we report (1)H, (13)C, and (15)N chemical shift assignments as determined by heteronuclear NMR spectroscopy .

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Galectin 3 / chemistry*
  • Humans
  • Nuclear Magnetic Resonance, Biomolecular*
  • Proline*
  • Recombinant Fusion Proteins / chemistry*

Substances

  • Galectin 3
  • Recombinant Fusion Proteins
  • Proline