Abstract
MicroRNAs (miRNAs) are small noncoding RNAs that have important roles in cancer. The altered expressions of miRNAs and their target genes are frequently detected in various tumors. In this study, downregulation of miR-15a-16 in nonsmall cell lung cancer (NSCLC) was found to be inversely correlated with Cripto. Results from the Luciferase reporter assay and Western blot analysis also confirmed that Cripto is a direct target of miR-15a-16. In addition, transfection of miR-15a-16 expression plasmid inhibited the invasion ability and promoted the apoptosis of NCI-H23 and NCI-H358 cells. Moreover, miR-15a-16 overexpression suppressed tumor growth in vivo. These findings clearly suggest that the downregulation of miR-15a-16 with Cripto amplification may be involved in the development of NSCLC.
MeSH terms
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3' Untranslated Regions / genetics
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Animals
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Apoptosis / genetics
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Base Sequence
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Carcinoma, Non-Small-Cell Lung / genetics*
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Carcinoma, Non-Small-Cell Lung / pathology*
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Cell Line, Tumor
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Cell Proliferation
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Disease Progression*
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GPI-Linked Proteins / genetics
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GPI-Linked Proteins / metabolism*
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Gene Expression Regulation, Neoplastic
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Humans
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / metabolism*
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Lung Neoplasms / genetics*
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Lung Neoplasms / pathology
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Mice, Inbred BALB C
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Mice, Nude
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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Molecular Sequence Data
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Neoplasm Invasiveness
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism*
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Protein Binding
Substances
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3' Untranslated Regions
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GPI-Linked Proteins
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Intercellular Signaling Peptides and Proteins
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MIRN15 microRNA, human
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MIRN16 microRNA, human
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MicroRNAs
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Neoplasm Proteins
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TDGF1 protein, human