Control of reactive oxygen species (ROS) production through histidine kinases in Aspergillus nidulans under different growth conditions

Saki Hayashi; Megumi Yoshioka; Tetsuji Matsui; Kensuke Kojima; Masashi Kato; Kyoko Kanamaru; Tetsuo Kobayashi

doi:10.1016/j.fob.2014.01.003

Control of reactive oxygen species (ROS) production through histidine kinases in Aspergillus nidulans under different growth conditions

FEBS Open Bio. 2014 Jan 7:4:90-5. doi: 10.1016/j.fob.2014.01.003. eCollection 2014.

Authors

Saki Hayashi¹, Megumi Yoshioka¹, Tetsuji Matsui¹, Kensuke Kojima¹, Masashi Kato¹, Kyoko Kanamaru¹, Tetsuo Kobayashi¹

Affiliation

¹ Laboratory of Applied Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.

Abstract

Sensor histidine kinases (HKs) are important factors that control cellular growth in response to environmental conditions. The expression of 15 HKs from Aspergillus nidulans was analyzed by quantitative real-time PCR under vegetative, asexual, and sexual growth conditions. Most HKs were highly expressed during asexual growth. All HK gene-disrupted strains produced reactive oxygen species (ROS). Three HKs are involved in the control of ROS: HysA was the most abundant under the restricted oxygen condition, NikA is involved in fungicide sensing, and FphA inhibits sexual development in response to red light. Phosphotransfer signal transduction via HysA is essential for ROS production control.

Keywords: Aspergillus nidulans; DTT, dithiothreitol; FGSC, Fungal Genetics Stock Center; GSH, glutathione; HK, histidine kinase; HPt, histidine-containing phosphotransfer; Histidine kinase; NBT, nitro-blue tetrazolium; Phosphotransfer; ROS, reactive oxygen species; RR, response regulator; Reactive oxygen species; Signal transduction; t-BOOH, tert-butyl hydroperoxide.