Abstract
Wnt signaling has been implicated in promoting somatic cell reprogramming. However, its molecular mechanisms remain unknown. Here we report that Wnt/β-catenin enhances iPSCs induction at the early stage of reprogramming. The augmented reprogramming induced by β-catenin is not due to increased total cell population or activation of c-Myc. In addition, β-catenin interacts with reprogramming factors Klf4, Oct4, and Sox2, further enhancing expression of pluripotency circuitry genes. These studies reveal novel mechanisms underlying the regulation of reprogramming somatic cells to pluripotency by Wnt/β-catenin signaling.
Keywords:
Beta-catenin; Gene Expression; Induced Pluripotent Stem (iPS) Cell; Reprogramming; Wnt Signaling; c-myc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Differentiation
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HEK293 Cells
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Humans
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Induced Pluripotent Stem Cells / cytology*
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Induced Pluripotent Stem Cells / metabolism
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Kruppel-Like Factor 4
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Kruppel-Like Transcription Factors / metabolism
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Mice
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Octamer Transcription Factor-3 / metabolism
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SOXB1 Transcription Factors / metabolism
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Signal Transduction*
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TCF Transcription Factors / metabolism
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Wnt Proteins / metabolism*
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beta Catenin / metabolism*
Substances
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KLF4 protein, human
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Klf4 protein, mouse
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Kruppel-Like Factor 4
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Kruppel-Like Transcription Factors
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Octamer Transcription Factor-3
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Pou5f1 protein, mouse
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SOXB1 Transcription Factors
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Sox2 protein, mouse
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TCF Transcription Factors
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Wnt Proteins
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beta Catenin