Reply to the article entitled "Identification of an 18 bp deletion in the TWIST1 gene by CO-amplification at lower denaturation temperature-PCR (COLD-PCR) for non-invasive prenatal diagnosis of craniosynostosis: first case report" by Galbiati et al., Clin Chem Lab Med 2014;52(4):505-9

MeSH terms

• Cold Temperature*
• Craniosynostoses / diagnosis*
• Female
• Humans
• Nuclear Proteins / genetics*
• Polymerase Chain Reaction / methods*
• Pregnancy
• Prenatal Diagnosis*
• Sequence Deletion / genetics*
• Twist-Related Protein 1 / genetics*