The bisegmented genome of a putative double-stranded (ds) RNA virus from Ustilaginoidea virens was sequenced and analyzed. The larger genomic segment of 2112 bp encodes a putative RNA-dependent RNA polymerase (RdRp, 628 aa), and the smaller one of 2082 bp encodes a putative coat protein (CP) of 539 aa. The 5' untranslated regions (UTR) of the two segments share regions of high sequence homology. Phylogenetic analysis indicates that this novel partitivirus, named Ustilaginoidea virens partitivirus 2 (UvPV2), can be assigned to the family Partitiviridae.