Intrinsic membrane proteins of the thylakoids of Chlamydomonas reinhardii

H P Michel; E Schneider; M Tellenbach; A Boschetti

doi:10.1007/BF00032359

Intrinsic membrane proteins of the thylakoids of Chlamydomonas reinhardii

Photosynth Res. 1981 Sep;2(3):203-12. doi: 10.1007/BF00032359.

Authors

H P Michel¹, E Schneider, M Tellenbach, A Boschetti

Affiliation

¹ Institut für Blochemie der Universität Bern, Freiestrasse 3, 3012, Bern, Switzerland.

PMID: 24470233
DOI: 10.1007/BF00032359

Abstract

Upon protoolytle treatment of thylakoid membranes, extrinsic proteins are selectively degraded. The remaining resistant proteins have been analyzed by SDS-PAGE. In the thylakoids of Chlamydomonas reinhardii, six polypeptides or protein fragments of 20 kD or higher are resistant to proteolysis. These intrinsic proteins have been identified as: the apoproteins of the chlorophyll-protein complexes CP I and LHCP; a polypeptide whose presence is related to the chlorophyll b content of the cells; and a portion of a chloroplast-made 34 kD polypeptide. Furthermore, after proteolytic treatment of the membranes, the LHCP complex can be resolved into two subcomplexes, apparently differing in their polypeptide composition.