Regulation of sodium influx and sodium, potassium pump activity by a sodium-hydrogen antiport in rat vascular smooth muscle

Clin Exp Pharmacol Physiol. 1987 Mar;14(3):191-5. doi: 10.1111/j.1440-1681.1987.tb00374.x.

Abstract

1. The effects of Na-H antiport inhibitors on sodium influx, intracellular pH and Na,K-pump activity were examined in vascular smooth muscle from rats. 2. Amiloride and 5-(N-ethyl-N-isopropyl)amiloride inhibited Na accumulation following inhibition of the Na,K-pump. 3. Inhibition of Na influx was associated with a reduction in basal Na,K-pump activity and intracellular pH. 4. It is concluded that a Na-H antiport system in vascular smooth muscle regulates Na influx rate, contributes to intracellular pH regulation and influences basal levels of Na,K-pump activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amiloride / pharmacology
  • Animals
  • Aorta
  • Cells, Cultured
  • Culture Techniques
  • Hydrogen-Ion Concentration
  • Intracellular Fluid / metabolism
  • Ion Channels*
  • Muscle, Smooth, Vascular / metabolism*
  • Ouabain / pharmacology
  • Potassium
  • Rats
  • Rats, Inbred Strains
  • Sodium
  • Spectrometry, Fluorescence

Substances

  • Ion Channels
  • Ouabain
  • Amiloride
  • Sodium
  • Potassium