Abstract
RNA sequences having up to three consecutive internal amide linkages were synthesized and studied using UV and NMR spectroscopy. The amide modifications did not interfere with normal base-pairing and A-type RNA conformation. Three consecutive amides were well tolerated in the passenger strand of siRNA and caused little change in RNAi activity.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amides / chemistry*
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Base Sequence
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HeLa Cells
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Humans
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Models, Molecular
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Nucleic Acid Conformation
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RNA Interference*
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RNA, Small Interfering / administration & dosage
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RNA, Small Interfering / chemical synthesis
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RNA, Small Interfering / chemistry*
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RNA, Small Interfering / genetics*
Substances
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Amides
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RNA, Small Interfering