Abstract
Human mesenchymal stem cells (hMSCs) remodel or regenerate various tissues through several mechanisms. Here, we identified the hMSC-secreted protein SCRG1 and its receptor BST1 as a positive regulator of self-renewal, migration, and osteogenic differentiation. SCRG1 and BST1 gene expression decreased during osteogenic differentiation of hMSCs. Intriguingly, SCRG1 maintained stem cell marker expression (Oct-4 and CD271/LNGFR) and the potentials of self-renewal, migration, and osteogenic differentiation, even at high passage numbers. Thus, the novel SCRG1/BST1 axis determines the fate of hMSCs by regulating their kinetic and differentiation potentials. Our findings provide a new perspective on methods for ex vivo expansion of hMSCs that maintain native stem cell potentials for bone-forming cell therapy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP-ribosyl Cyclase / genetics*
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ADP-ribosyl Cyclase / metabolism
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Antigens, CD / genetics*
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Antigens, CD / metabolism
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Cell Differentiation / genetics*
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Cell Movement / genetics
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Down-Regulation
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Focal Adhesion Protein-Tyrosine Kinases / metabolism
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GPI-Linked Proteins / genetics
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GPI-Linked Proteins / metabolism
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Gene Expression Regulation, Developmental
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Humans
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JNK Mitogen-Activated Protein Kinases / metabolism
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Mesenchymal Stem Cells / cytology
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Mesenchymal Stem Cells / metabolism*
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Osteogenesis / genetics*
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Phosphatidylinositol 3-Kinases / metabolism
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Protein Binding
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Protein Biosynthesis
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Signal Transduction
Substances
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Antigens, CD
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GPI-Linked Proteins
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Nerve Tissue Proteins
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SCRG1 protein, human
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Phosphatidylinositol 3-Kinases
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Focal Adhesion Protein-Tyrosine Kinases
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Extracellular Signal-Regulated MAP Kinases
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JNK Mitogen-Activated Protein Kinases
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ADP-ribosyl Cyclase
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ADP-ribosyl cyclase 2