Molecular cloning and expression of IRAK-4, IL-17 and I-κB genes in Haliotis rufescens challenged with Vibrio anguillarum

Valentina Valenzuela-Muñoz; Cristian Gallardo-Escárate

doi:10.1016/j.fsi.2013.12.015

Molecular cloning and expression of IRAK-4, IL-17 and I-κB genes in Haliotis rufescens challenged with Vibrio anguillarum

Fish Shellfish Immunol. 2014 Feb;36(2):503-9. doi: 10.1016/j.fsi.2013.12.015. Epub 2014 Jan 4.

Authors

Valentina Valenzuela-Muñoz¹, Cristian Gallardo-Escárate²

Affiliations

¹ Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P.O. Box 160-C, Concepción, Chile.
² Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P.O. Box 160-C, Concepción, Chile. Electronic address: crisgallardo@oceanografia.udec.cl.

PMID: 24398261
DOI: 10.1016/j.fsi.2013.12.015

Abstract

The candidate genes interleukin-1 receptor associated kinase 4 (IRAK-4), Interleukin 17 (IL-17) and Inhibitor of NF-κB (I-κB) were cloned and evaluated in Californian abalone (Haliotis rufescens) hemocytes in response to Vibrio anguillarum. Molecular characterization evidenced that HrI-κB has a full cDNA sequence of 3027 bp with an encoding region of 401 amino acids (aa), HrIRAK-4 comprised 1969 bp that encoded for 516 aa, and Hr-IL17 had a full sequence of 806 bp encoding for 165 aa. qPCR analysis showed the higher constitutive expression level of Hr-IL17 in hemocytes; meanwhile Hr-IκB and Hr-IRAK4 gene expression levels were higher in gills and mantle. The assessment of gene expression in hemocytes after infection with V. anguillarum evidences the immune responses of Hr-IκB, Hr-IRAK4, and Hr-IL17 and their relationships through the NF-κB signaling pathway.

Keywords: Haliotis rufescens; I-κB; IL-17; IRAK-4; Vibrio anguillarum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Gene Expression Regulation*
I-kappa B Proteins / chemistry
I-kappa B Proteins / genetics*
I-kappa B Proteins / metabolism
Immunity, Innate*
Interleukin-1 Receptor-Associated Kinases / chemistry
Interleukin-1 Receptor-Associated Kinases / genetics*
Interleukin-1 Receptor-Associated Kinases / metabolism
Interleukin-17 / chemistry
Interleukin-17 / genetics*
Interleukin-17 / metabolism
Molecular Sequence Data
Organ Specificity
Phylogeny
Real-Time Polymerase Chain Reaction / veterinary
Sequence Alignment
Snails / classification
Snails / genetics*
Snails / immunology
Snails / microbiology*
Vibrio / physiology

Substances

I-kappa B Proteins
Interleukin-17
Interleukin-1 Receptor-Associated Kinases

Associated data

GENBANK/KF357963
GENBANK/KF357964
GENBANK/KF357965