Erythroid-extrinsic regulation of normal erythropoiesis by retinoic acid receptors

Br J Haematol. 2014 Jan;164(2):280-5. doi: 10.1111/bjh.12578. Epub 2013 Sep 24.

Abstract

Vitamin A and its derivatives (retinoids) are important regulators of haematopoiesis, acting via retinoic acid receptors (RARs). Epidemiological studies indicated an association of vitamin A deficiency with anaemia in humans. To define the requirements of RARs in erythropoiesis, we evaluated erythroid parameters in RAR germ-line deficient and conditional knock out mice with erythroid specific deletion of RARs. Adult RARγ(-/-) mice were anaemic, however, Epor-Cre Rara(fl/fl) , Epor-Cre Rarg(fl/fl) and Epor-Cre Rara(fl/fl) g(fl/fl) mice were normal, indicating a lack of an erythroid intrinsic RAR function. Therefore, erythroid-specific RAR function is dispensable for erythropoiesis and RARγ plays an erythroid extrinsic role in erythropoiesis.

Keywords: anaemia; erythroid development; experimental haematology; retinoic acid; vitamin A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / metabolism
  • Erythroblasts / metabolism
  • Erythropoiesis / physiology*
  • Immunophenotyping
  • Mice
  • Mice, Knockout
  • Phenotype
  • Receptors, Erythropoietin / genetics
  • Receptors, Retinoic Acid / deficiency
  • Receptors, Retinoic Acid / genetics
  • Receptors, Retinoic Acid / physiology*
  • Retinoic Acid Receptor alpha
  • Retinoic Acid Receptor gamma

Substances

  • RARA protein, human
  • Rara protein, mouse
  • Receptors, Erythropoietin
  • Receptors, Retinoic Acid
  • Retinoic Acid Receptor alpha