Estimated albumin excretion rate versus urine albumin-creatinine ratio for the assessment of albuminuria: a diagnostic test study from the Prevention of Renal and Vascular Endstage Disease (PREVEND) Study

Am J Kidney Dis. 2014 Mar;63(3):415-21. doi: 10.1053/j.ajkd.2013.10.061. Epub 2013 Dec 21.

Abstract

Background: Albumin-creatinine ratio (ACR) in spot urine samples is recommended for albuminuria screening instead of measured albumin excretion rate (mAER) in 24-hour urine collections. In patients with extremes of muscle mass, differences in spot urine creatinine values may lead to under- or overestimation of mAER by ACR. We hypothesized that calculating estimated AER (eAER) using spot ACR and estimated creatinine excretion rate (eCER) may improve albuminuria assessment.

Study design: Diagnostic test study.

Setting & participants: 2,711 community-living individuals from the general population of the Netherlands participating in the PREVEND (Prevention of Renal and Vascular Endstage Disease) Study.

Index test: eAER was computed as the product of ACR and eCER. eCER was computed using 3 previously validated methods (Ix, Ellam, and Walser).

Reference test: mAER, based on two 24-hour urine collections. Accuracy of the eAER and ACR were defined as the percentage of participants falling within 30% (P30) of mAER.

Results: Mean age was 49 years, 46% were men, mean estimated glomerular filtration rate was 84 ± 15 mL/min/1.73 m(2), and median mAER was 7.2 (IQR, 5.4-11.0) mg/d. Mean measured CER was 1,381 mg/d, and median ACR was 4.9 mg/g. Using the Ix equation, median eAER was 6.4 mg/d. In the full cohort, eAER was more accurate and less biased compared to ACR (P30, 48.9% vs 33.6%; bias, -34.2% vs -14.1%, respectively). In subgroup analysis, improvement was most notable in the middle and highest weight tertiles and in men. Using the other methods for eCER produced similar results.

Limitations: Little ethnic heterogeneity and a generally healthy cohort make extension of findings to other races and the chronically ill uncertain.

Conclusions: In a large community-dwelling cohort, eAER was more accurate than ACR in assessing albuminuria.

Keywords: Albuminuria; albumin-creatinine ratio; creatinine excretion.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adult
  • Aged
  • Albuminuria / urine*
  • Biomarkers / urine
  • Creatinine / urine*
  • Female
  • Glomerular Filtration Rate
  • Humans
  • Kidney Failure, Chronic / diagnosis*
  • Kidney Failure, Chronic / prevention & control
  • Kidney Failure, Chronic / urine
  • Male
  • Middle Aged
  • Netherlands
  • Predictive Value of Tests
  • ROC Curve
  • Reproducibility of Results

Substances

  • Biomarkers
  • Creatinine