Staphylococcal enterotoxin C2 promotes osteogenesis and suppresses osteoclastogenesis of human mesenchymal stem cells

Exp Cell Res. 2014 Mar 10;322(1):202-7. doi: 10.1016/j.yexcr.2013.12.008. Epub 2013 Dec 16.

Abstract

As a super-antigen, staphylococcal enterotoxin C2 (SEC2) stimulates the release of massive inflammatory cytokines such as interferon-gamma (IFN-γ), interleukin-1 (IL-1) and interleukin-2 (IL-2) which are documented to implicate osteoblast differentiation. In the present study, SEC2 was found to significantly improve the osteoblast differentiation by up-regulating BMP2 and Runx2/Cbfa1 expression. Interferon (IFN)-inducible gene IFI16, a co-activator of Runx2/Cbfa1, was also activated by SEC2 in the osteoblast differentiation. In addition, exogenous introduction of SEC2 stimulated OPG expression and suppressed RANKL, suggesting suppression of osteoclastogenesis in hMSCs. Therefore, our results displayed that SEC2 plays an important role in the commitment of MSC to the osteoblast and it might be a potential new therapeutic candidate for bone regeneration.

Keywords: Human mesenchymal stem cell; Osteoblast; Osteoclast; Staphylococcal enterotoxin C2; Superantigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Regeneration / drug effects
  • Cell Differentiation / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Core Binding Factor Alpha 1 Subunit / genetics
  • Enterotoxins / pharmacology*
  • Gene Expression / drug effects
  • Humans
  • Mesenchymal Stem Cells / drug effects*
  • Mesenchymal Stem Cells / physiology
  • Nuclear Proteins / physiology
  • Osteoblasts / drug effects
  • Osteoblasts / physiology
  • Osteoclasts / drug effects*
  • Osteoclasts / physiology
  • Osteogenesis / drug effects*
  • Phosphoproteins / physiology

Substances

  • Core Binding Factor Alpha 1 Subunit
  • Enterotoxins
  • Nuclear Proteins
  • Phosphoproteins
  • RUNX2 protein, human
  • IFI16 protein, human
  • enterotoxin C, staphylococcal