Carbohydrate-functionalized collagen matrices: design and characterization of a novel neoglycosylated biomaterial

Carbohydr Res. 2014 May 7:389:12-7. doi: 10.1016/j.carres.2013.11.008. Epub 2013 Nov 17.

Abstract

Collagen matrices have been neoglycosylated with lactose by reductive amination at lysine side chains. AFM analysis highlights that the chemical does not affect molecular assembly into fibrils. Moreover, ELLA biochemical assays show that the glycan moiety is efficiently exposed on the matrix surface for receptor recognition.

Keywords: AFM; Collagen; Lactose; Lectins; Neoglycosylation; Reductive amination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry*
  • Carbohydrate Conformation
  • Collagen / chemistry*
  • Drug Design*
  • Glycosylation
  • Lactose / chemistry*
  • Molecular Dynamics Simulation

Substances

  • Biocompatible Materials
  • Collagen
  • Lactose