Abstract
Antigen preservation for presentation is a hallmark of potent antigen-presenting cells. In this paper, we report that in human macrophages and dendritic cells, a subset of phagosomes gets coated with Atg8/LC3, a component of the molecular machinery of macroautophagy, and maintains phagocytosed antigens for prolonged presentation on major histocompatibility complex class II molecules. These Atg8/LC3-positive phagosomes are formed around the antigen with TLR2 agonists and require reactive oxygen species production by NOX2 for their generation. A deficiency in the NOX2-dependent formation of these antigen storage phagosomes could contribute to compromise antifungal immune control in chronic granulomatous disease patients.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / metabolism*
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Antigen Presentation*
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Autophagy / physiology*
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Autophagy-Related Protein 8 Family
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Histocompatibility Antigens Class II / metabolism*
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Humans
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Membrane Glycoproteins / metabolism
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Membrane Glycoproteins / physiology
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Microfilament Proteins / metabolism*
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NADPH Oxidase 2
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NADPH Oxidases / metabolism
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NADPH Oxidases / physiology
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Phagosomes / metabolism*
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Phagosomes / physiology
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Reactive Oxygen Species / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Autophagy-Related Protein 8 Family
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GABARAPL2 protein, human
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Histocompatibility Antigens Class II
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Membrane Glycoproteins
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Microfilament Proteins
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Reactive Oxygen Species
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CYBB protein, human
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NADPH Oxidase 2
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NADPH Oxidases