Multicenter validation of the VITEK MS v2.0 MALDI-TOF mass spectrometry system for the identification of fastidious gram-negative bacteria

John A Branda; Jenna Rychert; Carey-Ann D Burnham; Maureen Bythrow; Omai B Garner; Christine C Ginocchio; Rebecca Jennemann; Michael A Lewinski; Ryhana Manji; A Brian Mochon; Gary W Procop; Sandra S Richter; Linda F Sercia; Lars F Westblade; Mary Jane Ferraro

doi:10.1016/j.diagmicrobio.2013.08.013

Multicenter validation of the VITEK MS v2.0 MALDI-TOF mass spectrometry system for the identification of fastidious gram-negative bacteria

Diagn Microbiol Infect Dis. 2014 Feb;78(2):129-31. doi: 10.1016/j.diagmicrobio.2013.08.013. Epub 2013 Dec 8.

Authors

Affiliations

¹ Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA. Electronic address: jbranda@partners.org.
² Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
³ Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
⁴ Department of Pathology and Laboratory Medicine, North Shore-LIJ Health System Laboratories, Lake Success, NY, USA.
⁵ Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
⁶ Department of Pathology and Laboratory Medicine, North Shore-LIJ Health System Laboratories, Lake Success, NY, USA; Department of Pathology and Laboratory Medicine, Hofstra North Shore-LIJ School of Medicine, Hempstead, NY, USA.
⁷ Barnes Jewish Hospital, St. Louis, MO, USA.
⁸ Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA.
⁹ Barnes Jewish Hospital, St. Louis, MO, USA; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA; Department of Pathology and Laboratory Medicine, Hofstra North Shore-LIJ School of Medicine, Hempstead, NY, USA.

PMID: 24321357
DOI: 10.1016/j.diagmicrobio.2013.08.013

Abstract

The VITEK MS v2.0 MALDI-TOF mass spectrometry system's performance in identifying fastidious gram-negative bacteria was evaluated in a multicenter study. Compared with the reference method (DNA sequencing), the VITEK MS system provided an accurate, species-level identification for 96% of 226 isolates; an additional 1% were accurately identified to the genus level.

Keywords: Fastidious; Gram-negative bacilli; Identification; MALDI-TOF MS; VITEK.

Publication types

Multicenter Study
Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Bacterial Typing Techniques / methods*
Bacterial Typing Techniques / standards
Gram-Negative Bacteria / classification*
Gram-Negative Bacteria / genetics
Gram-Negative Bacteria / isolation & purification
RNA, Bacterial
RNA, Ribosomal, 16S
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / standards

Substances

RNA, Bacterial
RNA, Ribosomal, 16S